Variation Report

SOP: Using the Variation Report in Patsnap Bio to Analyze Sequence Variations Objective This SOP explains how to access and interpret the Variation Report in Patsnap Bio after running a sequence search. It helps users quickly identify mutation hotspots, filter results by residue, and review variation details for a selected sequence range.

• After completing a sequence search, go to the results page.
  
  Locate the Variation Report button on the middle right side of the screen.
  
  Click Variation Report to open the analysis view.
• Use the interactive heat map to visualize sequence variation across your hit set.
  
  Scan the map to quickly identify mutation hotspots without reviewing sequences one by one.
  
  Focus on positions with the strongest variation signal to prioritize important mutations.
• Read the heat map color intensity to understand variation frequency.
  
  Darker colors indicate a higher number of variation instances at that position.
  
  Use this visual cue to determine which locations in the query sequence vary most across the results.
• Click on a residue in the heat map to isolate that position.
  
  Review the filtered result list to see only sequences with variation at the selected location.
  
  Use this filter to narrow analysis to the mutations most relevant to your research.
• If you only need to analyze part of the sequence, define the range of interest.
  
  Rerun the heat map analysis for that smaller segment.
  
  Use this approach when you want a more focused view of variation in a specific region.
• Review the table beneath the heat map for a position-by-position breakdown.
  
  Interpret each row as a summary of substitutions at a given location.
  
  Example interpretation:
  
  At location 46, one sequence has a proline replacing alanine.
  
  At the same location, 282 instances of glycine replace alanine.
  
  Use the table to validate what the heat map shows and to capture exact mutation counts.
• Do not rely on color alone; always confirm findings in the table below the heat map.
  
  Ensure the correct sequence search results are loaded before opening the Variation Report.
  
  When rerunning the report for a smaller range, verify the selected region matches your research question.
  
  A filtered residue view only shows sequences with variation at that position, so it may exclude sequences that are otherwise relevant.
• Start with the heat map to quickly identify high-variation positions before reviewing individual sequences.
  
  Use residue filtering to reduce the result set and focus on the most relevant mutations.
  
  Narrow the sequence range when you only need insight into a specific domain or region.
  
  Use the table to capture exact substitution counts for reporting or downstream analysis.