The sequence variation filter is a pre and post-search filter that helps you to hit and screen the target sequence more accurately by allowing for position and residue-specific filtering. This is where you can start to look at replacements, insertions, and deletions, and pinpoint at which point, or multiple points, in that sequence you want that mutation to be in. A specific subsequence may be locked down and other regions varied, or a range containing multiple non-specific replacements may be studied.
- Alignment Region: this is where you can enter the start and end positions of the alignment and specify the target position inside the alignment region.
- Alignment Length: this is where you can fill in the starting and ending position information, the maximum length, and the minimum length information.
- Point Mutation: this is where you can enter point position information and alternative residues. Point position refers to a single point, such as 20, 30, etc., while replacement residues refer to the entered characters that can be replaced. You can add point mutations by clicking the + button.
Sample Workflow: to filter down on specific sequences with a variation
Let's say, after conducting a sequence search, from the variation report, we find that we are interested in a specific mutation. As an example, 5 Replacement V5L(8) means that the V at location 5 has been replaced by an L residue in 8 sequences.
Therefore, we would have to go back to the platform search results, click on ‘Variation Filter’ on the left-hand side, and select Point Mutation, Residue L, Location 5 as in the below screenshot:
This will then show the sequences that have this specific mutation:
The next step from here to view associated patents is to click the 'View Sources' button on the top right of the screen and then go into Analytics.